G9a, Recombinant Human, His-tagged Enzyme Description: Recombinant human G9a protein, (EHMT2 BAT8, KMT1C, accession Q96KQ7, amino acids 913-1210), containing an N-terminal 6xHis tag, expressed in E. coli. G9a is a SET-domain containing Histone methyltransferase, catalyzing the mono- and di-methylation of Histone H3 at lysine 9.
G9a, Recombinant Human, His-tagged Enzyme Formulation: Recombinant 6xHis-G9a in 100mM Tris pH 8.0, 10 mM glutathione and 25% glycerol.
Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Application Notes: G9a, Recombinant Human, His-tagged is useful for Histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 1-3 µg G9a per reaction with nucleosomes or recombinant Histone H3 as a substrate is recommended. Dilute as needed before using.
References: Kuo AJ et al (2011). Mol Cell 44: 609-620.
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Protein Gel Data: G9a, Recombinant Human, His-tagged, human (1 µg) run on a PAGE gel and stained with Coomassie blue. Migration and molecular weight of protein standards is indicated. (Click image to enlarge)
Enzyme Activity Data: G9a, Recombinant Human, His-Tagged (0.1 µg) was used in an HMTase assay with 0.25 µg HeLa Polynucleosomes (Cat. No. 16-0003) using a standard radiometric filter binding assay protocol. (Click image to enlarge)