SKU: 18-4112
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc1-2-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"658","bulk_discount_rates":[],"can_purchase":true,"meta_description":"","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/658/947/epidyne-remodeling-assay-substrate-dna-st601-gatc12-biotinylated__78976.1645734539.jpg?c=2","alt":"EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=658","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"577","name":"Pack Size","value":"50 µg"}],"sku":"18-4112","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 12pt;\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong>EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Description</strong></span></span>:</strong><span style=\"font-family: Arial; font-size: 12pt;\"> EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated is a 217 base-pair double-stranded DNA fragment containing a 5' biotin-TEG group. This sequence includes the Lowary 601 nucleosome positioning sequence (see 18-0005) as well as a 3' acceptor sequence to accomodate the histone octamer subsequent to remodeling. ST601-GATC1,2, Biotinylated DNA has a restriction enzyme sites embedded in the 601 sequence that are accessible after nucleosome remodeling.<br /><br /></span> </td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong>EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated </strong></span></span> Formulation:</strong> 50 µg lyophilyzed ST601-GATC1,2, Biotinylated DNA.<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong>EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Storage and Stability:</strong> Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.</span></span><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><br /> <br /> </span></span></p> </td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong><strong>EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated </strong> Application Notes:</strong> EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated is useful as a control for restriction enzyme accessibility nucleosome remodeling assays using the EpiDyne Remodeling Assay Substrate, as a DpnII restriction enzyme site is present within the 601 sequence.\n\n <br />\n <br />\n <strong>DNA Sequence:</strong> GAATTCATCAGAATCCCGGTGCCGAGGCC<span style=\"color:#FF0000;\">GATC</span>AATT<span style=\"color:#FF0000;\">GATC</span>GTAGACAGCTCTAGCACCGCTTAA<br />\nACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCAC<br />\nGTGTCAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGATGGATGATGATGGATG<br />\nAATAGATGGATGGATGAAGCTT<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br /> <span style=\"font-size: 10pt;\"> Lowary PT and J Widom (1998). <em>J Mol Biol</em> 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a></span><br /> </span></p> </td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/18-4112.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"18-4112 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> <p></p> </td> </tr> </tbody> </table> </td> <td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/18-4112-DNA-gel.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/18-4112-DNA-gel.jpg\" alt=\"18-4112 Gel Data\" width=\"241\" height=\"400\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>DNA Gel Data:</strong> Lane 1: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated (200ng) and Lane2: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated (200 ng) digested with 10U DpnII incubated at 37C for 1 hour resolved via native PAGE. Migration positions of DNA molecular weight markers are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </div>\n\n\n\n</body>\n</html>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$115.00","value":115,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"options":[],"related_products":[{"id":656,"sku":"16-4112","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-2-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":"\n\n\n \n\n \n\n \n\n\n\n EpiDyne Nucleosome 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Pack Size: 50 µg
