EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated

$545.00
SKU: 16-4114
Pack Size: 50 μg
EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by provided 263 base pair DNA sequence that includes the Widom 601 sequence with an added GATC and a 5' biotin-TEG group.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated Formulation: Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 271,084.4 Da.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66 Biotinylated Application Notes: This product is a template for nucleosome remodeling assays using the restriction enzyme DpnII to determine accessibility of GATCs which are masked in its native configuration (prior to remodeling).
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View technical datasheet for this product. 16-4114 Datasheet

16-4114 Protein Gel
DNA Gel Data: Free DNA (DNA, 200 ng) and EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated (Nuc, 400 ng) resolved via native PAGE and stained with ethidium bromide to visualize DNA.
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16-4114 DNA Gel
Protein Gel Data: Coomassie stained PAGE gel of proteins in Histone Proteins in EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66 Biotinylated resolved via SDS-PAGE and coomassie staining (2 µg) . Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.
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16-4114 Western Blot
Nucleosome Remodeling Data: ACF/ATP-dependent nucleosome remodeling reaction in the presence of DpnII restriction enzyme. EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66 Biotinylated nucleosomes incubated with (10 nM) or without ACF for up to 9 minutes in the presence of 2 mM ATP (or No ATP, as indicated) and 50U of DpnII. Samples were quenched at specified intervals and resolved via native PAGE.
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