Ultra-sensitive. Rapid results. Rigorously tested.
Cleavage Under Targets and Tagmentation (CUT&Tag) assays use a fusion of protein A, protein G, and Tn5 (pAG-Tn5) to selectively target antibody-labelled chromatin for high-resolution sequencing analysis. These innovations enable low-cost mapping from small numbers of cells – poising CUT&Tag to enable epigenomic studies of unprecedented breadth and scale.
EpiCypher is a leading provider of user-friendly CUT&Tag reagents and workflows. The CUTANA™ CUT&Tag Kit is the result of our rigorous optimization efforts and has been intentionally designed for rapid throughput and accessible pricing. Advantages include:
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Request More Info- Streamlined two-day protocol with no library prep
- Exclusive single-tube workflow maximizes yield
- Reliable profiling using low cell numbers
- Dramatically reduced assay costs
- Comprehensive kit goes from cells to sequence-ready DNA
Robust signal in active and repressed chromatin
CUTANA™ CUT&Tag maps histone PTMs at active regulatory elements (H3K4me1) and promoters (H3K4me3), constitutive (H3K9me3) and facultative (H3K27me3) heterochromatin and active gene bodies (H3K36me2/me3) in K562 cells. CUT&Tag is not recommended for chromatin-associated proteins.
High-quality chromatin mapping from low cell numbers
The CUTANA™ CUT&Tag protocol generates data with high signal over background using as few as 10,000 cells and only 5-8 million sequencing reads.
These advances are driven by EpiCypher’s one-tube CUT&Tag workflow, which minimizes sample loss and increases throughput. Combined with low sequencing depth requirements, our kit provides substantial cost savings vs. ChIP-seq.
Reliable workflow supported by robust controls
EpiCypher scientists have developed a user-friendly protocol that is highly consistent across assay replicates. The CUTANA™ CUT&Tag Kit comes with all the controls and guidance you need for successful experiments:
- Control antibodies
- Nucleosome spike-in controls
- Detailed quality control metrics
- Troubleshooting tips
Compatible with diverse sample processing conditions
The CUTANA™ CUT&Tag Kit was used to map H3K4me3 in native (unfixed) nuclei, native cells, and lightly cross-linked nuclei, using protocol adaptations outlined in the manual. Results are consistent across sample preparations, expanding the application of CUT&Tag to additional sample types and targets.
Fast-track your chromatin profiling:
Save time and costs with CUTANA™ CUT&Tag Kits
CUTANA™ CUT&Tag Kit – A Look Inside the Box!
Everything you need for high-quality chromatin mapping
