Chicken Polynucleosomes, Purified (HSW) Description: Polynucleosomes purified from chicken erythrocytes using a high salt wash (HSW). The nucleosome is the basic subunit of chromatin consisting of the histone octamer (two each of the four core histones, H2A, H2B, H3, and H4) wrapped by 147 base pairs of DNA. As shown by the size of the DNA species, Chicken Polyucleosomes are predominantly trimers, tetramers, and pentamers, and due to the HSW do not contain histone H1.
Chicken Polynucleosomes, Purified (HSW) Formulation: Purified Chicken Polynucleosomes in 10 mM Tris-HCl pH 8.0, 1 mM EDTA and 10% glycerol
Chicken Polynucleosomes, Purified (HSW) Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Chicken Polynucleosomes, Purified (HSW) Application Notes: Chicken Polynucleosomes are suitable for use in enzyme assays such as acetylation, methylation or phosphorylation, chromatin binding assays, or for use as a positive control in Western blotting.
References: Adhvaryu KK et al (2005). Eukaryot Cell 4: 1455-1564. Link Kizer KO et al (2005). Mol Cell Biol 25: 3305-3316. Link Morris SA et al (2005). Eukaryot Cell 4: 1446-1454. Link
View technical datasheet for this product.
DNA Gel Data: DNA was purified from Chicken Oligoucleosomes (1 µg) and run on an agarose gel to show the size of nucleosomal DNA compared to molecular weight markers (base pairs). Note that mononucleosomal DNA runs at roughly 150 base pairs. (Click image to enlarge)
Protein Gel Data: Coomassie stained PAGE gel of proteins in Chicken Polynucleosomes (2 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated. (Click image to enlarge)
Histone Methyltransferase Data: Chicken Polynucleosomes were used in a radioactive histone methyltransferase assay with recombinant G9a. Radioactive SAM was incubated with Chicken Mononucleosmes in the absence (-) or presence (+) of recombinant G9a and run on an acrylamide gel. HMTase activity co-purifying with the nucleosomes is undetectable. Left Top: Coomassie stained gel. Left Bottom: autoradiogram of gel. Right: Densitometry quantification of autoradiogram. (Click image to enlarge)