Recombinant CBP Bromodomain Description: Recombinant human CBP Bromodomain (CREB Binding protein, CREBBP, accession number Q92793, amino acids 1091 to 1190) containing an N-terminal GST tag, expressed in E. coli. CBP is an important regulator of cell function and serves to integrate a number of key cell signaling pathways. CBP contains intrinsic histone acetylatransferase (HAT) activity and serves as a co-activator for a large number of transcription factors. The bromodomain of CBP specifically recognizes a number of acetylated proteins, including histones and transcription factors. The CBP gene is mutated in Rubinestein-Taybi Syndrome, a rare developmental disorder.
Recombinant CBP Bromodomain Formulation: Recombinant GST-tagged protein at 0.37 mg/ml in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 20% glycerol.
Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Application Notes: Recombinant CBP Bromodomain is useful for protein binding and screening experiments examining acetylated protein substrates.
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Protein Gel Data: CBP Bromodomain (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. (Click image to enlarge)