SKU: 16-1014
{"url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-h2az-1-human-recombinant","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","options":[],"id":"518","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Human Recombinant H2AZ.1 Nucleosomes for Enzyme Screening Assays","category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)","Nucleosomes/Variant Nucleosomes (vNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/518/365/Histone_variant_nucleosome__17270.1523638379.gif?c=2","alt":"Mononucleosomes (H2AZ.1), Human Recombinant"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=518","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"249","name":"Pack Size","value":"50 μg"}],"sku":"16-1014","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody>\n\n<tr valign=\"top\"> \n\n<td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> \n\n\n\n<tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AZ.1), Human Recombinant Description: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes assembled from recombinant human histones expressed in <em>E. coli</em> (two each of histones H2AZ.1, H2B, H3 and H4. Accession numbers: H2AZ.1-P0C0S5; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. H2AZ.1 and H2AZ.2 are variants of histone H2A and implicated in diverse cellular functions ranging from transcriptional regulation, chromosome transmission and DNA damage repair. H2AZ has a dedicated deposition machinery (the SWR-C ATPase).</span><br /> \n <br /></p></td> \n</tr> <tr> \n \n \n \n\n <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AZ.1), Human Recombinant Formulation: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Purified recombinant mononucleosomes (50 µg total mass, 27.4 µg in 50.0 µl) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol. Nucleosome molecular weight = 198,425.5 Da.<br /> <br /> </span></span></td> \n </tr> \n \n \n \n <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AZ.1), Human Recombinant </strong><strong>Storage and Stability:</strong> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. <br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AZ.1), Human Recombinant </strong><strong>Application Notes:</strong> Mononucleosomes (H2AZ.1), Human Recombinant are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. <strong>EpiCypher <strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AZ.1), Human Recombinant </strong>do not contain free DNA which could alter assayed activities.</strong><br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br />\n <br /> <br /> </span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-1014.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-1014 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> </tbody> </table> </td> \n \n \n \n <td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr>\n <td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-1014-protein-gel-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-1014-protein-gel-data.jpg\" alt=\"16-1014 Protein Gel Data\" width=\"150\" height=\"325\" /></a> </td></tr><tr>\n <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data: </strong>Coomassie stained PAGE gel of proteins in Nucleosome (H2AZ.1), Human Recombinant (1 µg) demonstrates the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2AZ.1, H2B, H3 and H4) are indicated. <br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr>\n <td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-1014-DNA-gel-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-1014-DNA-gel-data.jpg\" alt=\"16-1014 DNA Gel Data\" width=\"150\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome (H2AZ.1), Human Recombinant resolved via native PAGE and stained with ethidium bromide to visualize DNA. Lane 1: DNA extracted from nucleosomes (100 ng). Lane 2: Intact nucleosomes (400 ng).<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr>\n <td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-1014-WB-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-1014-WB-data.jpg\" alt=\"16-1014 Protein Gel Data\" width=\"150\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong> Western Analysis of Nucleosome (H2AZ.1), Human Recombinant. Top Panel: Wild type (Lane 1) and H2AZ.1-containing nucleosomes (Lane 2) were probed with an anti-H2AZ antibody and analyzed via ECL readout. Only the H2AZ.1 samples produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing histones from H2A (Lane 1) and H2AZ.1 <br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> \n \n \n \n </td> </tr> </tbody> </table> </div>\n\n \n\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Human Recombinant H2AZ.1 Nucleosomes","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"Mononucleosomes (H2AZ.1), Human Recombinant, SWR-C, H2AZ, H2AZ.1, 16-1014, vnuc","show_quantity_input":1,"title":"Mononucleosomes (H2AZ.1), Human Recombinant","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/518/365/Histone_variant_nucleosome__17270.1523638379.gif?c=2","alt":"Mononucleosomes (H2AZ.1), Human Recombinant"}]}
Pack Size: 50 μg
