SKU: 16-0027
{"url":"https://www.epicypher.com/products/nucleosomes/recombinant-nucleosomes-rnucs/tailless-nucleosomes-recombinant-human-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"732","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Tailless Nucleosomes, Recombinant Human, Biotinylated for Use as Enzyme Assay Substrates","category":["Nucleosomes/Recombinant Nucleosomes (rNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/732/685/Human_recombinant_nucleosome__14471.1515789856.1280.1280__38618.1566413775.1280.1280__12756.1581105654.jpg?c=2","alt":"Tailless Nucleosomes, Recombinant Human, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=732","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"484","name":"Pack Size","value":"50 μg"}],"sku":"16-0027","description":"<body>\n\n\n\n\n<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody>\n\n\n\n<tr valign=\"top\"> \n\n\n\n<td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> \n\n\n\n\n\n\n\n<tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 11pt;\">Tailless Nucleosomes, Human Recombinant, Biotinylated</strong><strong style=\"font-family: Arial; font-size: 11pt;\"> Description: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 11pt;\">Mononucleosomes assembled from recombinant human histones expressed in <i>E. coli</i> (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805). All four of the histones have had their tails enzymatically removed after wrapping by 147 base pairs of 601 positioning sequence DNA. There is a 5’ biotin-TEG group on the DNA which makes these nucleosomes ideal for use as a negative control in binding assays and pull-down experiments. The nucleosome is the basic subunit of chromatin. </span><br /> \n <br />\n \n </td> \n</tr> <tr> \n \n \n \n \n \n \n \n \n \n \n <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 11pt;\">Tailless Nucleosomes, Human Recombinant, Biotinylated Formulation: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 11pt;\">Purified recombinant tailless nucleosomes (50 µg total mass protein+DNA, 25 µg protein) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol. <br /> <br /> </span></span></td> \n </tr> \n \n \n \n \n \n \n \n \n \n <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 11pt;\"><strong style=\"font-family: Arial; font-size: 11pt;\">Tailless Nucleosomes, Human Recombinant, Biotinylated</strong><strong> Storage and Stability:</strong> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. <br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 11pt;\"><strong style=\"font-family: Arial; font-size: 11pt;\">Tailless Nucleosomes, Human Recombinant, Biotinylated</strong><strong> Application Notes:</strong> Tailless Nucleosomes, Human Recombinant, Biotinylated are highly purified and are suitable for use as a negative control or substrate in enzyme screening assays or for nucleosome binding experiments. The absence of histone post-translational modifications makes them ideal for conducting enzyme activity and screening assays. The biotin group on the DNA makes pull-down experiments possible, allowing isolation of the nucleosomes after assay completion. <strong>EpiCypher <strong style=\"font-family: Arial; font-size: 11pt;\">Tailless Nucleosomes, Human Recombinant, Biotinylated</strong> do not contain free DNA which could alter assayed activities.</strong> </span></span><br /><br /> </td> </tr> \n\t\n\n\t<tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 11pt;\">References:</strong><br /> \n \n \n <span style=\"font-size: 10pt;\"><span style=\"font-family: Arial;\">Strelow JM <em>et al</em> (2016) <em>J Biomol Screen</em> 21: 786-794. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/27369108\" target=\"_new\">Link</a></span></span>\n \n \n \n \n <span style=\"font-size: 10pt;\"><span style=\"font-family: Arial;\"><br />\n Munari F <em>et al</em> (2012) <em>J Biol Chem</em> 287: 33756-33765. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/22815475\" target=\"_new\">Link</a></span></span>\n \n \n \n \n <span style=\"font-size: 10pt;\"><br />\n Luger K et al (1999) <em>Methods Mol Biol</em> 119: 1-16. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/10804500\" target=\"_new\">Link</a><br />\n Lowary PT and J Widom (1998) <em>J Mol Bio</em>l 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a><br />\n Ausio J <i>et al</i> (1989) <i>J Mol Biol</i> 206(3): 451-63. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/2716057\" target = \"_new\">Link</a></span><br />\n <br /> <br /> </span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 11pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-0027.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-0027 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> </tbody> </table> </td> \n \n \n \n <td> <table width=\"350\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16_0027_protein_gel.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16_0027_protein_gel.jpg\" alt=\"16-0027 SDS-PAGE\" width=\"281\" height=\"300\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><strong style=\"font-family: Arial; font-size: 10pt;\">Protein Gel Data:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Coomassie stained SDS-PAGE to demonstrate purity of the histones in the preparation. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Lane 1</strong><span style=\"font-family: Arial; font-size: 10pt;\"> shows histone proteins in the nucleosome before enzymatic removal of tails. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Lane 2</strong><span style=\"font-family: Arial; font-size: 10pt;\"> shows the resulting histone proteins after tail removal. Size of molecular weight markers and positions of the intact core histones (H2A, H2B, H3, and H4) are indicated. </span><strong style=\"font-family: Arial; font-size: 10pt;\"><br />\n Click image to enlarge.\n </strong></td> </tr> \n \n \n \n \n <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16_0027_dna_gel.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16_0027_dna_gel.jpg\" alt=\"16-0027 Native PAGE\" width=\"225\" height=\"302\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>DNA Gel Data:</strong> Recombinant Nucleosomes resolved via native PAGE and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (100ng). <strong>Lane 2:</strong> Intact Nucleosomes (400ng). <strong>Lane 3:</strong> Tailless Nucleosomes after enzymatic removal of histone tails.<br />\n <strong style=\"font-family: Arial; font-size: 10pt;\">Click image to enlarge.</strong> </span></span></td> </tr> \n \n \n \n \n </tbody> </table> </td> </tr> </tbody> </table> </div>\n\n\n\n\n\n\n</body>\n</html>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Tailless Nucleosomes, Recombinant Human, Biotinylated (16-0027)","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"options":[],"related_products":[{"id":677,"sku":"16-0384","name":"Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k27bu-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified 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Pack Size: 50 μg
