SKU: 13-0040
{"url":"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me1-antibody-snap-chip-certified","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"warranty":"","gtin":"","max_purchase_quantity":0,"id":"716","can_purchase":false,"meta_description":"","category":[],"meta_keywords":"","AddThisServiceButtonMeta":"","images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/716/671/snap-chip-ab__70507.1557259520.1280.1280__90586.1575485337.png?c=2","alt":"Histone H3K4me1 Antibody, SNAP-ChIP Certified *DISCONTINUED*"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/716/763/h3k4me1-epicypher-13-0040__63932.1606315328.jpg?c=2","alt":"Histone H3K4me1 Antibody, SNAP-ChIP Certified *DISCONTINUED*"}],"main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/716/671/snap-chip-ab__70507.1557259520.1280.1280__90586.1575485337.png?c=2","alt":"Histone H3K4me1 Antibody, SNAP-ChIP Certified *DISCONTINUED*"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=716","shipping":[],"num_reviews":0,"weight":"0.10 LBS","custom_fields":[{"id":"1129","name":"Pack Size","value":"100 µg"}],"sku":"13-0040","description":"<table cellpadding=\"2\" cellspacing=\"2\" style=\"width: 100%\">\n <tbody>\n <tr valign=\"top\">\n <a\n style=\"color: #fff\"\n href=\"/products/antibodies/cut-and-run-antibodies/cut-and-run-antibodies-histone-ptms/h3k4me1-antibody-snap-certified-for-cut-and-run-and-cut-and-tag\">\n <p\n style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n margin-top: 2.5rem;\n \">\n Product Discontinued - See 13-0057 as a recommended alternative\n </p>\n </a>\n <td>\n <table cellpadding=\"1\" cellspacing=\"1\" style=\"width: 95%\">\n <tbody>\n <tr>\n <td>\n <table cellpadding=\"0\" cellspacing=\"0\" style=\"width: 100%\">\n <tbody>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Type: </strong>Mixed\n Monoclonal<sup>*</sup></span\n >\n </td>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Host: </strong>Rabbit</span\n >\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Mol Wgt.: </strong>15 kDa</span\n >\n </td>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Appl.: </strong>ChIP, ChIP-Seq,\n WB, ICC, E, L</span\n >\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Format: </strong>Aff. Pur.\n IgG</span\n >\n </td>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Reactivity: </strong>H, M, WR</span\n >\n </td>\n </tr>\n </tbody>\n </table>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong\n >Histone H3K4me1 Antibody, SNAP-ChIP Certified\n Description:</strong\n >\n This antibody meets EpiCypher’s “<a\n href=\"https://www.epicypher.com/technologies/snap-chip-spike-ins/certified-antibodies\"\n >SNAP-ChIP<sup>®</sup> Certified</a\n >” criteria for specificity and efficient target\n enrichment in a ChIP experiment (<20% cross-reactivity\n across the panel, >5% recovery of target input). Histone H3\n is one of the four proteins that are present in the\n nucleosome, the basic repeating subunit of chromatin,\n consisting of 147 base pairs of DNA wrapped around an octamer\n of core histone proteins (H2A, H2B, H3 and H4). This antibody\n reacts to H3K4me1 when present alone as well as in combination\n with nearby acetylations (tetraAc-H3). No cross reactivity\n with extended acyl states (H3K9bu, H3K9cr) or other lysine\n acylations in the EpiCypher SNAP-ChIP K-AcylStat panel, is\n detected. <br /><sup>*</sup>Mixed Monoclonal: a pool of\n multiple monoclonal antibodies.</span\n ><br />\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong\n >Histone H3K4me1 Antibody, SNAP-ChIP Certified Certified\n Immunogen:</strong\n >\n A synthetic peptide corresponding to histone H3 monomethylated\n at lysine 4.</span\n >\n <p></p>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong\n >Histone H3K4me1 Antibody, SNAP-ChIP Certified Certified\n Formulation:</strong\n >\n Protein A affinity-purified antibody (1 mg/mL) in PBS, pH 7.2\n with 0.09% sodium azide</span\n >\n <p></p>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong\n >Histone H3K4me1 Antibody, SNAP-ChIP Certified Certified\n Storage and Stability:</strong\n >\n Stable for 1 years at -20°C from date of receipt.</span\n >\n <p></p>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong\n >Histone H3K4me1 Antibody, SNAP-ChIP Certified Certified\n Application Notes</strong\n >\n <span style=\"font-weight: normal\"\n ><strong>Recommended dilutions:</strong><br />\n </span\n ><strong>ChIP</strong>: 2 - 5 μg per 1x10<sup>6</sup> cells\n <strong>IF/ICC</strong>: 1 μg/mL <strong>WB</strong>: 0.5 - 1\n μg/mL <strong>L</strong>: 0.5 - 2 μg/mL</span\n >\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><span style=\"font-size: 12pt\"\n ><strong\n >Histone H3K4me1 Antibody, SNAP-ChIP Certified Certified\n References:</strong\n ></span\n ></span\n >\n </p>\n\n <p></p>\n\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n >Grzybowski et al (2015) Mol Cell 58:886</span\n >\n </p>\n\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n >Shah et al (2018) Mol Cell 72:162</span\n >\n </p>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n >View technical datasheet for this product.\n <a\n href=\"https://www.epicypher.com/content/documents/tds/13-0040.pdf\"\n target=\"_new\">\n <img\n alt=\"13-0040 Datasheet\"\n height=\"40\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\"\n width=\"30\" /></a\n ></span>\n </td>\n </tr>\n <tr>\n <td>\n <p></p>\n\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><span style=\"font-size: 10pt\"\n ><strong>Applications Key: ChIP</strong>-Chromatin\n IP; <strong>E</strong>-ELISA; <strong>FACS</strong>-Flow\n cytometry; <strong>IF</strong>-Immunofluorescence;\n <strong>IHC</strong>-Immunohistochemistry;\n <strong>ICC</strong>-Immunocytochemistry;\n <strong>L</strong>-Luminex;\n <strong>IP</strong>-Immunoprecipitation;\n <strong>WB</strong>-Western Blotting</span\n ></span\n >\n </p>\n\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><span style=\"font-size: 10pt\"\n ><strong>Reactivity Key: </strong\n ><strong>B</strong>-Bovine; <strong>Ce</strong>-C.\n elegans; <strong>Ch</strong>-Chicken; <strong>Dm</strong>-\n Drosophila; <strong>Eu</strong>-Eukaryote;\n <strong>H</strong>-Human; <strong>M</strong>-Mouse;\n <strong>Ma</strong>-Mammal; <strong>R</strong>-Rat;\n <strong>Sc</strong>-S.cerevesiae; <strong>Sp</strong>-S.\n pombe; <strong>WR</strong>-Wide Range (predicted);\n <strong>X</strong>-Xenopus;\n <strong>Z</strong>-Zebrafish</span\n ></span\n >\n </p>\n </td>\n </tr>\n </tbody>\n </table>\n </td>\n <td>\n <table cellpadding=\"3\" cellspacing=\"3\" style=\"width: 300px\">\n <tbody>\n <tr>\n <td>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><a\n href=\"https://cdn11.bigcommerce.com/s-y9o92/product_images/uploaded_images/h3k4me1-epicypher-13-0040.jpg?t=1600893742&_ga=2.132898317.241922123.1600715432-402741698.1598987696\"\n target=\"_new\"\n ><img\n alt=\"13-0040 SNAP-ChIP Data\"\n height=\"100\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/product_images/uploaded_images/h3k4me1-epicypher-13-0040.jpg?t=1600893742&_ga=2.132898317.241922123.1600715432-402741698.1598987696\"\n width=\"150\" /></a\n ></span>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Representative SNAP-ChIP-seq results: </strong>\n Cumulative histogram plot and heatmap of signal intensity\n depict H3K4me1 ChIP-seq data aligned to annotated\n transcription start sites (TSS, +/- 3.0 kb; left). Two\n representative genomic regions depicting H3K4me1 peak\n structure and distribution are shown (right). Data shown are\n representative of H3K4me1 ChIP antibody (EpiCypher Catalog\n No. 13-0040) and are not lot-specific. Native ChIP-seq was\n performed using K562 cells as described\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/30244833/\"\n target=\"_blank\"\n >(Shah et al., Mol Cell 2018)</a\n >\n with SNAP-ChIP<sup>TM</sup> K-MetStat Spike-in (Catalog No.\n 19-1001) nucleosome controls added prior to chromatin\n digestion to confirm antibody specificity and ChIP\n efficiency. Paired-end sequencing libraries were prepared\n using the NEBNext<sup>®</sup> Ultra<sup>TM</sup> II DNA\n Library Prep Kit for Illumina<sup>®</sup>. ChIP libraries\n were sequenced on an Illumina<sup>®</sup> NextSeq.\n Sequencing reads were aligned to the human genome using\n Bowtie 2 (Johns Hopkins University). Bigwig files of read\n enrichment in binned genomic regions (signal intensity)\n flanking the indicated gene features were used to create a\n cumulative histogram plot and heatmap of signal intensity\n <a href=\"https://basepairtech.com/\" target=\"_blank\">\n (www.basepairtech.com)</a\n >. Gene browser shots were generated using the Integrative\n Genomics Viewer (IGV, Broad Institute) with the window size\n denoted (top).<br />\n <strong>(Click image to enlarge) </strong></span\n >\n </p>\n\n <p></p>\n </td>\n </tr>\n <tr>\n <td>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><a\n href=\"https://www.epicypher.com/content/images/products/antibodies/13_0040_SNAP.jpg\"\n target=\"_new\"\n ><img\n alt=\"13-0040 SNAP-ChIP Data\"\n height=\"100\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13_0040_SNAP.jpg\"\n width=\"150\" /></a\n ></span>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>SNAP-ChIP qPCR Data: </strong>Histone H3K4me1\n antibody (5 μg) was tested in a native ChIP experiment\n using chromatin from K-562 cells (3 μg) with the\n SNAP-ChIP K-AcylStat Panel (EpiCypher Catalog No. 19-3001)\n spiked-in prior to micrococcal nuclease digestion.\n Specificity (left Y-axis)was determined by qPCR for the DNA\n barcodes corresponding to modified nucleosomes in the\n SNAP-ChIP panel (x-axis). Black bar represents antibody\n efficiency (right y-axis; log scale) and indicates\n percentage of the target immunoprecipitated relative to\n input.<br />\n <strong>(Click image to enlarge) </strong></span\n >\n </p>\n\n <p></p>\n </td>\n </tr>\n <!--BEGIN IMAGE AND CAPTION SECTION-->\n <tr>\n <td>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><a\n href=\"https://www.epicypher.com/content/images/products/antibodies/13_0040_luminex.jpg\"\n target=\"_new\"\n ><img\n alt=\"13-0040 Luminex Data\"\n height=\"100\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13_0040_luminex.jpg\"\n width=\"200\" /></a\n ></span>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Luminex Data:</strong> Histone H3K4me1 antibody was\n assessed using a Luminex® based approach employing\n dCypher™ Nucleosome K-MetStat Panel (EpiCypher Catalog No.\n 16-9001). The panel comprises biotinylated designer\n nucleosomes (X-Axis) individually coupled to uniquely\n identifiable Luminex Magplex® beads. Antibody binding to\n nucleosomes were tested in multiplex (24-plex) at a 1:1000\n dilution, and detected with second layer anti-IgG*PE. Data\n was generated using a Luminex FlexMAP3D®. Data\n normalized to relevant on-target (H3K4me1; set to 100) is\n shown. H<br />\n <strong>(Click image to enlarge) </strong></span\n >\n </p>\n\n <p></p>\n </td>\n </tr>\n <!--END IMAGE AND CAPTION SECTION-->\n <tr valign=\"top\">\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><a\n href=\"https://www.epicypher.com/content/images/products/antibodies/13_0040_wb_tds.jpg\"\n target=\"_new\"\n ><img\n alt=\"13-0040 Western Blot data\"\n height=\"200\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13_0040_wb_tds.jpg\"\n width=\"85\" /></a\n ></span>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Western Blot Data: </strong>Western blot of acid\n extracts from HeLa cells untreated ( - ) or treated with\n sodium butyrate ( + ), using EpiCypher Catalog No. 13-0040\n at 0.25 µg/mL.<br />\n <strong>(Click image to enlarge) </strong></span\n >\n </p>\n\n <p></p>\n </td>\n </tr>\n <tr valign=\"top\">\n <td valign=\"top\">\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><a\n href=\"https://www.epicypher.com/content/images/products/antibodies/13_0040_IFICC_tds.jpg\"\n target=\"_new\"\n ><img\n alt=\"13-0040 ICC\"\n height=\"100\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13_0040_IFICC_tds.jpg\"\n width=\"127\" /></a\n ></span>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <p>\n <span style=\"font-family: arial, helvetica, sans-serif\"\n ><strong>Immunocytochemistry Data: </strong\n >Immunocytochemistry of HeLa cells treated with sodium\n butyrate, using H3K4me1 antibody, EpiCypher Catalog No.\n 13-0040 (red). Actin filaments have been labeled with\n fluorescein phalloidin (green).<br />\n <strong>(Click image to enlarge) </strong></span\n >\n </p>\n\n <p></p>\n </td>\n </tr>\n </tbody>\n </table>\n </td>\n </tr>\n </tbody>\n</table>\n\n<style>\n .button-group {\n display: none;\n }\n</style>\n","tags":[],"detail_messages":"","availability":"","page_title":"","mpn":"","upc":null,"options":[],"related_products":[{"id":626,"sku":"13-0028","name":"Histone H3K4me3 Antibody, SNAP-ChIP Certified *DISCONTINUED*","url":"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified","availability":"","rating":null,"brand":{"name":null},"category":[],"summary":"\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/626/578/Screen_Shot_2018-09-25_at_2.00.27_PM__52796.1538135192.png?c=2","alt":"Histone H3K4me3 Antibody, SNAP-ChIP Certified *DISCONTINUED*"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/626/578/Screen_Shot_2018-09-25_at_2.00.27_PM__52796.1538135192.png?c=2","alt":"Histone H3K4me3 Antibody, SNAP-ChIP Certified *DISCONTINUED*"}],"date_added":"24th Sep 2018","pre_order":false,"show_cart_action":false,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":452,"name":"Pack Size","value":"100 μg"}],"num_reviews":null,"weight":{"formatted":"0.00 LBS","value":0},"demo":false,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=626"},{"id":759,"sku":"13-0045","name":"Histone H3K27ac Antibody, SNAP-ChIP® Certified *DISCONTINUED*","url":"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k27ac-antibody-snap-chip-certified","availability":"","rating":null,"brand":{"name":null},"category":[],"summary":"\n \n Product Discontinued -\n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/759/741/1579731877.1280.1280__62293.1580846736.1280.1280__62794.1592491196.png?c=2","alt":"Histone H3K27ac Antibody, SNAP-ChIP® Certified *DISCONTINUED*"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/759/741/1579731877.1280.1280__62293.1580846736.1280.1280__62794.1592491196.png?c=2","alt":"Histone H3K27ac Antibody, SNAP-ChIP® Certified *DISCONTINUED*"}],"date_added":"17th Jun 2020","pre_order":false,"show_cart_action":false,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":517,"name":"Pack size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=759"},{"id":878,"sku":"13-0050","name":"Histone H3K18acyl Antibody, SNAP-ChIP® Certified","url":"https://www.epicypher.com/products/antibodies/snap-chip-sup-sup-certified-antibodies/histone-h3k18acyl-antibody-snap-chip-certified","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/SNAP-ChIP<sup>®</sup> Certified Antibodies"],"summary":"\n \n \n Type: Polyclonal\n \n \n Target Size: 15 kDa\n \n \n Format: Affi","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/878/905/snap-chip-ab__70507.1557259520.1280.1280__90586.1575485337.190.250__92644.1626671512.190.250__94652.1628650844.png?c=2","alt":"Histone H3K18acyl Antibody, SNAP-ChIP® Certified"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/878/905/snap-chip-ab__70507.1557259520.1280.1280__90586.1575485337.190.250__92644.1626671512.190.250__94652.1628650844.png?c=2","alt":"Histone H3K18acyl Antibody, SNAP-ChIP® Certified"}],"date_added":"11th Aug 2021","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":932,"name":"Pack Size","value":"100 µL"}],"num_reviews":null,"weight":{"formatted":"0.00 LBS","value":0},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=878","price":{"without_tax":{"currency":"USD","formatted":"$515.00","value":515},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=878"},{"id":690,"sku":"13-0037","name":"Histone H4K12ac Antibody - 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Pack Size: 100 µg
