SETD2, Recombinant Human GST-tagged Enzyme Description: SETD2, Recombinant Human GST-tagged protein (SET2, HYPB, HIF1, KMT3A, accession Q9BYW2, amino acids 1345-1711), containing an N-terminal GST tag, expressed in E. coli. SETD2 is a SET-domain containing histone methyltransferase, catalyzing the trimethylation of histone H3 at lysine 36. SETD2 is a candidate tumor suppressor protein and mutated in clear cell renal cell carcinoma.
SETD2, Recombinant Human GST-tagged Enzyme Formulation: Recombinant SETD2, GST-tagged (1 µg/µl) in 50mM Tris pH 7.3, 300 mM NaCl, 4mM DTT, 1µM ZnCl2 and 25% glycerol.
Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Application Notes: SETD2, Recombinant Human GST-tagged is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 0.5 - 2.5 µg SETD2 per reaction with nucleosomes or recombinant histone H3 as a substrate is recommended.
References: Hacker KE et al (2016). J Biol Chem DOI: 10.1074/jbc.M116.739375
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Protein Gel Data: SETD2, Recombinant Human GST-tagged, human (1 µg) run on a PAGE gel and stained with Coomassie blue. Migration and molecular weight of protein standards is indicated. (Click image to enlarge)
Enzyme Activity Data: SETD2, Recombinant Human GST-tagged (500 nmol) was used in an HMTase assay with 1 µg Chicken Polynucleosomes (Cat. No. 16-0004) using a standard radiometric filter binding assay protocol. (Click image to enlarge)