|Type: Polyclonal ||Host: Rabbit |
|Mol Wgt.: 250 kDa ||Appl.: WB |
|Format: Aff. Pur. IgG ||Reactivity: H |
SETD2 Antibody Description: SETD2 (SET2, HYPB, HIF1, KMT3A, accession Q9BYW2, amino acids 1345-1711) is a SET-domain containing histone methyltransferase, catalyzing the trimethylation of histone H3 at lysine 36. SETD2 is a candidate tumor suppressor protein and mutated in clear cell renal cell carcinoma.
|SETD2 Antibody Immunogen: Recombinant protein corresponding to amino acids amino acids 1345-1711 of human SETD2. |
|SETD2 Antibody Formulation: Immunoaffinity purified rabbit IgG in PBS (pH 7.4) with 0.02% sodium azide and 1% BSA. |
|Storage and Stability: Stable for 2 years at -20°C from date of receipt. |
|Application Notes / Recommended dilutions: |
WB 1:500 - 1:2,000
|View technical datasheet for this product. |
View additional specificity data for this product.
Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting
Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish
Western Blot Data: Western blot using SETD2 Antibody (1:500 dilution). Lane 1: Whole cell extract (50,000 whole cell equivalents) from HKC cells transformed with a random shRNA construct.
Lane 2: Whole cell extract (50,000 whole cell equivalents) from HKC cells transformed with construct stably expressing an shRNA targeting SETD2.
(Click image to enlarge)