PRMT5 (protein arginine methyltransferase 5, HRMT1L5, IBP72, JBP1, SKB1, accession O14744) is a type II argininine methyltransferase, an enzyme that catalyzes the formation of monomethylarginine and symmetric dimethylarginine. PRMT5 methylates histone H3 Arg8, H4 Arg3, severl PIWI proteins, HOXA9, and a variety of proteins invovled in cell cycle regulation and the DNA damage signaling pathway, including p53.
PRMT5 Antibody Immunogen:
Synthetic peptide corresponding to amino acids 60-79 of human PRMT5, conjugated to KLH.
PRMT5 Antibody Formulation:
Rabbit serum with 30% glycerol and 0.035% sodium azide.
PRMT5 Antibody Storage and Stability:
Stable for 2 years at -20°C from date of receipt.
PRMT5 Antibody Application Notes:
PRMT5 antibody is useful for immunoprecipitation (5-10 µl per IP) and Western blotting (1:500 - 1:2,000 dilution).
PRMT5 Antibody References:
Boisvert FM et al (2002) J Cell Biol 159: 957-969. Link Gonsalvez GB et al (2007). J Cell Biol 178: 733-740. Link Yu Z et al (2009). Mol Cell Biol 29: 2982-2996. Link
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Western Blot Data: Western blot using PRMT5 Antibody (1:500 dilution) on mouse embryonic fibroblast (MEF) whole cell extract derived from cells with (+/+) or without (-/-) the PRMT5 gene. The band corresponding to the PRMT5 protein (indicated by an arrow) is absent in the PRMT5 null cells. The relative migration positions and molecular weights of protein size standards are indicated, as well as a Western blot recognizing the ?-actin protein, a loading control. (Click image to enlarge)