SKU: 16-0006
{"url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-human-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","options":[],"id":"84","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Highly Purified Recombinant Human Biotinylated Nucleosomes for Enzyme Assays, Screening and Kinetics","category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/84/331/Human_recombinant_nucleosome__14471.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=84","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1251","name":"Pack Size","value":"50 μg"},{"id":"1252","name":"Internal Comment","value":"Has Bulk - Not Included in Amount"},{"id":"1253","name":"Internal Comment","value":"65 extra units located bottom-right of Psylocke"}],"sku":"16-0006","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\"\n >\n <p>\n Mononucleosomes assembled from recombinant human histones expressed\n in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4.\n Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805)\n wrapped by 147 base pairs of 601 positioning sequence DNA. There is\n a 5’ biotin-TEG group on the DNA which makes them ideal for use in\n nucleosome binding assays and pull-down experiments. The nucleosome\n is the basic subunit of chromatin.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16_0006_dna_web.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0006 DNA Gel\"\n src=\"/content/images/products/nucleosomes/16_0006_dna_web.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA Gel Data </strong\n ><br />Mononucleosomes, Human Recombinant Biotinylated run\n on an agarose gel and stained with ethidium bromide to\n visualize DNA. <strong>Lane 1: </strong>Intact nucleosomes\n (400 ng).\n <strong\n >Lane 2: DNA extracted from nucleosomes (100 ng).</strong\n >\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16_0006_protein_web.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0006 Protein Gel\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16_0006_protein_web.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein Gel Data </strong><br />\n Coomassie stained PAGE gel of proteins in Mononucleosomes,\n Human Recombinant Biotinylated (0.5 µg) to demonstrate\n the purity and integrity of the histones in the preparation.\n Sizes of molecular weight markers and positions of the core\n histones (H2A, H2B, H3 and H4) are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/SET8_data.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n >\n <img loading=\"lazy\"\n alt=\"16-0006 Protein Gel\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/SET8_data.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong\n >Figure 3: Histone Methyltransferase Assay Data </strong\n ><br />\n Recombinant SET8 histone methyltransferase was used in\n radioactive methyltransferase assays with a variety of\n substrates as indicated, with SET8 exhibiting the highest\n level of activity in combination with recombinant\n nucleosomes (rNucs).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"16-0006 DNA Gel\"\n src=\"/content/images/products/nucleosomes/16_0006_dna_web.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"16-0006 Protein Gel\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16_0006_protein_web.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"16-0006 Protein Gel\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/SET8_data.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <!-- <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n \n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to human SNF2L amino acids\n 1004 to 1054.\n </div>\n </div> -->\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Purified recombinant mononucleosomes (50 µg total mass\n protein+DNA, 27.3 µg protein) in 10 mM Tris-HCl pH 7.5, 1 mM\n EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -20°C from date of receipt. For\n best results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n <strong>Application Notes Addendum:</strong> Mononucleosomes, Human\n Recombinant Biotinylated are highly purified and are suitable for\n use as substrates in enzyme screening assays or for nucleosome\n binding experiments. The absence of post-translational histone\n modifications makes them ideal for conducting enzyme activity and\n screening assays. The biotin group on the DNA makes pull-down\n experiments possible, allowing you to isolate the nucleosomes after\n your assay is complete.\n <strong\n >EpiCypher Mononucleosomes, Recombinant Human Biotinylated do not\n contain free DNA which could alter assayed activities.</strong\n >\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n Luger <em>et al.</em> <em>Methods Mol. Biol.</em> (1999) PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/10804500/\"\n title=\"Expression and Purification of Recombinant Histones and Nucleosome Reconstitution\"\n target=\"new\"\n >\n 10804500</a\n ><br />\n Lowary & Widom <em>J Mol. Biol.</em> (1998) PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n title=\"New DNA Sequence Rules for High Affinity Binding to Histone Octamer and Sequence-Directed Nucleosome Positioning\"\n target=\"new\"\n >9514715</a\n ><br />\n <br />\n <strong>Product References:</strong>\n <iframe\n class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shr7JlhY6Hprkx5yD?backgroundColor=purple&viewControls=on\"\n frameborder=\"0\"\n onmousewheel=\"\"\n width=\"100%\"\n height=\"300\"\n style=\"background: transparent; border: 1px solid #ccc\"\n ></iframe>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-0006.pdf\"\n target=\"_new\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"15-1016 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Highly Purified Recombinant Human Biotinylated Nucleosomes for Enzyme Assays, Screening and Kinetics","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":"25 µg","shipping_messages":[],"rating":0,"meta_keywords":"Highly Purified Recombinant Nucleosomes for Enzyme Assays, Screening and Kinetics, recombinant nucleosome, recombinant chromatin, recombinant mononucleosomes, highly purified recombinant nucleosomes, human, biotinylated, 16-0006, chromatin, nucleosome, mononucleosome","show_quantity_input":1,"title":"Mononucleosomes, Recombinant Human Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/84/331/Human_recombinant_nucleosome__14471.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human Biotinylated"}]}
Pack Size: 50 μg
