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EpiCypher

Nucleosome, Recombinant Human, H3 K27me1 dNuc

Cat. No.: 16-0338
List Price: $549.00
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Product Description


Nucleosome, Recombinant Human, H3K27me1 dNuc Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary synthetic method) contains monomethyl-lysine at position 27. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.

Nucleosome, Recombinant Human, H3K27me1 dNuc Formulation: Nucleosome, Recombinant Human, H3K27me1 (27.3 µg protein weight, 50.0 µg DNA+protein) in 62.5 µl 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 3.98 μmolar. MW = 200,980.

Nucleosome, Recombinant Human, H3K27me1 dNuc Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Nucleosome, Recombinant Human, H3K27me1 dNuc Application Notes: Nucleosome, Recombinant Human, H3K27me1 dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K27me1 from EpiCypher does not contain free DNA which could alter assayed activities.

References:


View technical datasheet for this product. 16-0338 Datasheet
16-0338 Western Blot
Western Blot Data: Western Analysis of Nucleosome, Recombinant Human, H3K27me1. Top Panel: Unmodified H3 (Lane 1) and H3K27me1 containing nucleosomes (Lane 2) were probed with an anti-H3K27me1 antibody and analyzed via ECL readout. Only the H3K27me1 sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H3K27me1 nucleosomes (Lane 2).
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16-0338 MS Data
Mass Spec Data: Synthetic H3K27me1 protein analyzed by high resolution mass spectrometry. Expected mass = 15,286.9 Da. Determined mass = 15,287.26 Da.
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16-0338 Protein Gel Data
Protein Gel Data: Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H3K27me1 (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K27me1 and H4) are indicated.
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16-0338 Protein Gel Data
DNA Gel Data: Nucleosome, Recombinant Human, H3K27me1 run on a native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA extracted from nucleosomes (200 ng). Lane 2: Intact nucleosomes (400 ng).
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