Nucleosome Assembly 601 Sequence DNA, Biotinylated Description: Nucleosome Assembly 601 Sequence DNA, Biotinylated is a 147 base-pair double-stranded DNA fragment that was identified by Lowary and Widom (1998) using the SELEX method. The 601 sequence DNA has high affinity for histone octamers and is useful for in vitro nucleosome assembly. There is a biotin group on the 5' end of the fragment, which makes it ideal for use in nucleosome binding assays and pull-down experiments.
This product supersedes Catalog No. 18-0001 as a direct replacement.
Nucleosome Assembly Sequence 601 DNA, Biotinylated Formulation: 50 µg lyophilyzed 601 sequence DNA containing a biotin group on the 5' end.
Nucleosome Assembly Sequence 601 DNA, Biotinylated Storage and Stability: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.
Nucleosome Assembly Sequence 601 DNA, Biotinylated Application Notes: Nucleosome Assembly 601 Sequence DNA, Biotinylated is useful for assembly of nucleosomes using purified or recombinant histone octamers (Catalog No. 16-0005). The biotin group on the DNA allows for pull-down of the nucleosomes for nucleosome binding experiments or enzymatic assays. See website for more information and protocols.
References: Lowary PT and J Widom (1998). J Mol Biol 276: 19-42. Link
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Nucleosome core particle reconstitution protocol.
DNA Gel Data: Nucleosome Assembly Sequence 601 DNA, Biotinylated (200 ng) run on a 1.8% TBE agarose gel. Migration positions of DNA molecular weight markers are indicated. (Click image to enlarge)