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EpiCypher

NSD2 / MMSET Catalytic Domain, Recombinant Human

Cat. No.: 15-1002
List Price: $299.00
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Product Description

Type:  HMT Host:  E. coli
Mol Wgt.:  61 kDa Epitope Tag:  GST

NSD2 / MMSET Catalytic Domain, Recombinant Human Description: NSD2 / MMSET Catalytic Domain, Recombinant Human (WHSC1, accession O96028, amino acids 959-1365), containing an N-terminal GST tag, expressed in E. coli. NSD2/MMSET is a SET-domain containing histone methyltransferase, catalyzing the dimethylation of histone H3 at lysine 36. NSD2/MMSET, an oncogene that is overexpressed in several cancers, is thought to drive pathogenesis of t(4;14) positive multiple myeloma.

N.B.-Recombinant NSD2/MMSET requires nucleosomal substrates for activity.

NSD2 / MMSET Catalytic Domain, Recombinant Human Formulation: GST-NSD2/MMSET (0.5 µg/µl) in 25 mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT, 1 mM EDTA and 20% glycerol.

NSD2 / MMSET Catalytic Domain, Recombinant Human Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

NSD2 / MMSET Catalytic Domain, Recombinant Human Application Notes: NSD2 / MMSET Catalytic Domain, Recombinant Human is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 0.5 - 2 µg NSD2 per reaction with HeLa or recombinant nucleosomes as a substrate is recommended.

NSD2 / MMSET Catalytic Domain, Recombinant Human References:
Kuo AJ et al (2011). Mol Cell 44: 609-620. Link


View technical datasheet for this product. 15-1002 Datasheet
New!! View NSD2 AlphaNuc non-radioactive assay protocol 15-1002 Datasheet
15-1002 Protein Gel Data

Protein Gel Data: NSD2 / MMSET Catalytic Domain, Recombinant Human (1 µg) run on a PAGE gel and stained with Coomassie blue. Migration and molecular weights of protein standards are indicated.
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15-1002 Enzyme Activity Data

Enzyme Activity Data: NSD2 / MMSET Catalytic Domain, Recombinant Human was used in a radioactive methylation assay with either 0.5 μM recombinant nucleosomes or 0.5 μM recombinant histone octamer as the substrate. The reaction was spotted on filter paper and the radioactivity incorporated was counted (CPM). Varying amounts of enzyme were used, as indicated.
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