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EpiCypher

Mononucleosomes (H3.3 K27M), Recombinant Human Biotinylated

Cat. No.: 16-1323
List Price: $449.00
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Product Description

Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a methionine at position 27, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of M for K at position 27 in human H3.1 and H3.3 is a dominant negative inhibitor of the EZH2 methytransferase complex PRC2 and associated with pediatric brain cancer (DIPG). The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequece that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.

Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated Formulation: Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 242,900 Da.

Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated Application Notes: This product is a substrate for chromatin remodeling assays using restriction enzymes to determine accessibility of GATC subsequent to chromatin remodeling.
Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylateddo not contain free DNA which could alter assayed activities.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC References:

View technical datasheet for this product. 16-1323 Datasheet

16-1323 Western Blot
Western Blot Data: Coomassie stained PAGE gel of proteins in Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated (2 µg) demonstrates the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.
16-1323 DNA Gel
DNA Gel Data: Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated run using native PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA extracted from nucleosomes (200 ng). Lane 2: Intact nucleosomes (400 ng). Note absence of free DNA in "Nuc", indicating all DNA complexed with histones.
16-1323 Protein Gel
Protein Gel Data: Coomassie stained PAGE gel of proteins in Mononucleosomes (H3.3K27M), Recombinant Human (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3.3K27M and H4) are indicated.