|Histone Octamer, Recombinant Human Description: Human histone octamers (two each of histones H2A, H2B, H3 and H4) made from recombinant histones expressed in E. coli (accession numbers: H2A-P0C0S8; H2B-P62807; H3-P68431; H4-P62805). The histone octamer is the protein component of the nucleosome, the basic subunit of chromatin. A nucleosome consists of a histone octamer wrapped with 147 base pairs of DNA. |
|Histone Octamer, Recombinant Human Formulation: Gel filtration-purified recombinant human histone octamers in 20 mM Tris pH 7.5, 2 M NaCl, 1 mM EDTA, 5 mM 2-mercaptoethanol. |
|Histone Octamer, Recombinant Human Storage and Stability: Stable for 6 months at -20°C from date of receipt. For best results, aliquot and avoid repeated freezing and thawing. |
|Histone Octamer, Recombinant Human Application Notes: Human recombinant histone octamers can be used for chromatin reconstitution experiments, or as substrates for histone modifying enzymes. See links below to protocols for chromatin reconstitution. |
Cao XJ et al (2013). Epigenetics 8: 477 - 485.
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Nucleosome core particle reconstitution protocol.