Histone Octamer, Recombinant Human

SKU: 16-0001
Pack Size: 50 μg

Histone Octamer, Recombinant Human Description: Human histone octamers (two each of histones H2A, H2B, H3 and H4) made from recombinant histones expressed in E. coli (accession numbers: H2A-P0C0S8; H2B-P62807; H3-P68431; H4-P62805). The histone octamer is the protein component of the nucleosome, the basic subunit of chromatin. A nucleosome consists of a histone octamer wrapped with 147 base pairs of DNA.

Histone Octamer, Recombinant Human Formulation: Gel filtration-purified recombinant human histone octamers in 20 mM Tris pH 7.5, 2 M NaCl, 1 mM EDTA, 5 mM 2-mercaptoethanol.

Histone Octamer, Recombinant Human Storage and Stability: Stable for 6 months at -20°C from date of receipt. For best results, aliquot and avoid repeated freezing and thawing.

Histone Octamer, Recombinant Human Application Notes: Human recombinant histone octamers can be used for chromatin reconstitution experiments, or as substrates for histone modifying enzymes. See links below to protocols for chromatin reconstitution.

Cao XJ et al (2013). Epigenetics 8: 477 - 485.

View technical datasheet for this product. 16-0001 Datasheet

Nucleosome core particle reconstitution protocol. 16-0001 Datasheet

16-0001 Gel
Protein Gel Data: Histone Octamer, Recombinant Human (1 µg) run on a PAGE gel and stained with Coomassie blue to demonstrate the purity of the preparation. The positions of the individual histones are indicated.
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16-0001 Methylation Data
Methylation Assay Data: Histone Octamer, Recombinant Human used in methylation assays with GST-tagged SET domains as indicated. Reactions were loaded onto a PAGE gel and subjected to Western blotting. Top panel: anti-H3K9me2. Bottom panel: anti-histone H3 C-term (Catalog No. 13-0001).
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16-0001 Purification

Purification Data: Chromatogram from gel filtration purification of Histone Octamer, Recombinant Human. Only fractions corresponding to the octamers as indicated were collected and pooled.
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50 μg