Histone Octamer, Recombinant Human Description: Human histone octamers (two each of histones H2A, H2B, H3 and H4) made from recombinant histones expressed in E. coli (accession numbers: H2A-P0C0S8; H2B-P62807; H3-P68431; H4-P62805). The histone octamer is the protein component of the nucleosome, the basic subunit of chromatin. A nucleosome consists of a histone octamer wrapped with 147 base pairs of DNA.
Histone Octamer, Recombinant Human Formulation: Gel filtration-purified recombinant human histone octamers in 20 mM Tris pH 7.5, 2 M NaCl, 1 mM EDTA, 5 mM 2-mercaptoethanol.
Histone Octamer, Recombinant Human Storage and Stability: Stable for 6 months at -20°C from date of receipt. For best results, aliquot and avoid repeated freezing and thawing.
Histone Octamer, Recombinant Human Application Notes: Human recombinant histone octamers can be used for chromatin reconstitution experiments, or as substrates for histone modifying enzymes. See links below to protocols for chromatin reconstitution.
References: Cao XJ et al (2013). Epigenetics 8: 477 - 485.
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Nucleosome core particle reconstitution protocol.
Protein Gel Data: Histone Octamer, Recombinant Human (1 µg) run on a PAGE gel and stained with Coomassie blue to demonstrate the purity of the preparation. The positions of the individual histones are indicated. Click to enlarge image.
Methylation Assay Data: Histone Octamer, Recombinant Human used in methylation assays with GST-tagged SET domains as indicated. Reactions were loaded onto a PAGE gel and subjected to Western blotting. Top panel: anti-H3K9me2. Bottom panel: anti-histone H3 C-term (Catalog No. 13-0001). Click to enlarge image.
Purification Data: Chromatogram from gel filtration purification of Histone Octamer, Recombinant Human. Only fractions corresponding to the octamers as indicated were collected and pooled. Click to enlarge image.