Histone H4 R3me2s Peptide, Biotinylated Description: A synthetic peptide derived from human histone H4, amino acids 1-23, symmetrically dimethylated at arginine 3. The alpha-amino group on the N-terminus is acetylated, and there is an added C-terminal lysine residue containing a biotin moiety at the epsilon amino group, and a C-terminal amide group (CONH2).
Purity: >80% as judged by HPLC and mass spectrometry.
Storage and Stability: Lyophilized peptides are stable for 2 years at -20°C from date of shipment. Peptides in solution are stable for 6 months at -80°C. Spin vial briefly in microfuge and reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot peptide to avoid repeated freezing and thawing.
Quality Control: Peptide purified by reversed-phase HPLC and analyzed using MALD-TOF mass spectrometry and analytical RP-HPLC.
Mass Spec Data: MALDI-TOF mass spectrometry was used to analyze Histone H4 R3me2s Peptide, Biotinylated for identity and homogeneity.