Rabbit polyclonal antibody recognizing the histone H3 symmetrically dimethylated at arginine 8. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Symmetric dimethylation of arginine 8 by PRMT5 is associated with transcriptional repression and elevated in lymphoid cancer cells.
Histone H3 R8Me2s Antibody Immunogen:
Synthetic peptide corresponding to a region of human histone H3 symmetrically dimethylated at arginine 8.
Histone H3 R8Me2s Antibody Formulation:
Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Histone H3 R8Me2s Antibody Quality Control:
This antibody has been validated for use in IF, IHC, IP and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
Is specific for H3 R8Me2s
Has no cross-reactivity to related modifications
Is unaffected by nearby modifications
Histone H3 R8Me2s Antibody Storage and Stability:
Stable for 2 years at -20°C from date of receipt.
Histone H3 R8Me2s Antibody Application Notes
Recommended dilutions: IF/IHC 1:50 - 1:200 IP 2 - 5 µg per IP WB 1:500 - 1:2,000
Histone H3 R8Me2s AntibodyReferences:
View technical datasheet for this product. View additional specificity data for this product.
Peptide Array Specificity Data: EpiTitan™ Histone Peptide Array used to analyze histone modification specificity of Histone H3 R8Me2s Antibody (Arg8 symmetric dimethyl) catalog number 13-0019. Antibody signal appears in red, while the spotting tracer appears in green. (Click image to enlarge)
Specificity Data-Array: EpiTitan™ Histone Peptide arrays used to analyze the histone modification specificity of Histone H3 R8Me2s Antibody. Data is shown for the highest reacting singly-modified peptides. For more specificity data, see Additional Peptide Array Specificity Data. (Click image to enlarge)
Specificity Data-Dot Blot: Peptide dot blot used to confirm the specificity of Histone H3 R8Me2s Antibody. Methylated peptides corresponding to the immunogen and related sites were spotted onto PVDF and probed with the antibody. (Click image to enlarge)
Immunofluorescence Data: HEK293T cells stained with Histone H3R8Me2s Antibody (red) and counterstained with DAPI (blue). (Click image to enlarge)
Western Blot Data: HeLa cell extract (HeLa) and recombinant histone H3 (H3 protein) were blotted onto PVDF and probed with Histone H3 R8Me2s Antibody. (Click image to enlarge)