|Type: Polyclonal ||Host: Rabbit |
|Mol Wgt.: 15 kDa ||Appl.: ChIP, IF, IHC, WB |
|Format: Aff. Pur IgG ||Reactivity: H, WR |
Histone H3 R8Me1 Antibody Description: Rabbit polyclonal antibody recognizing the histone H3 monomethylated at arginine 8. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Monomethylation of arginine 8 is catalyzed by PRMT2 and PRMT5.
Histone H3 R8Me1 Antibody Immunogen: Synthetic peptide corresponding to a region of human histone H3 monomethylated at arginine 8.
Histone H3 R8Me1 Antibody Formulation:Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Histone H3 R8Me1 Antibody Quality Control:This antibody has been validated for use in IF, IHC, IP and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
- Is specific for H3 R8Me1
- Has no cross-reactivity to related modifications
- Is unaffected by nearby modifications
Histone H3 R8Me1 Antibody Storage and Stability:Stable for 2 years at -20°C from date of receipt.
Histone H3 R8Me1 Antibody Application Notes
ChIP 2 - 5 µg per IP
IF/IHC 1:50 - 1:200
WB 1:500 - 1:2,000
Histone H3 R8Me1 Antibody References:
|View technical datasheet for this product. |
View additional specificity data for this product.
Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting
Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish