Histone H3 K9Me1 Peptide Description: A synthetic peptide derived from human histone H3.1, amino acids 1-20, monomethylated at lysine 9, with an added C-terminal lysine residue containing a biotin moiety at the epsilon amino group, and a C-terminal amide group (CONH2).
Histone H3 K9Me1 Peptide Formulation: 50 µg of lyophilized powder.
Purity: >95% as judged by HPLC and mass spectrometry.
Storage and Stability: Lyophilized peptides are stable for 2 years at -20°C from date of shipment. Peptides in solution are stable for 6 months at -80°C. Spin vial briefly in microfuge and reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot peptide to avoid repeated freezing and thawing.
Quality Control: Peptide purified by reversed-phase HPLC and analyzed using MALD-TOF mass spectrometry and analytical RP-HPLC.
Mass Spec Data: MALDI-TOF mass spectrometry was used to analyze histone H3 K9Me1 peptide for identity and homogeneity.