Histone H3 K4Me2 Antibody Description: Rabbit polyclonal antibody recognizing the histone H3 dimethylated at lysine 4. Histone H3 is one of the core components of the nucleosome, the smallest subunit of chromatin. Dimethylation of histone H3 at lysine 4 (H3 K4Me2) by SET1, ASH1 or the MLL family of histone methyltransferases (HMTases) is associated with transcriptional activation and also frequently found in the context of H3 acetylation at multiple residues.
Histone H3 K4Me2 Antibody Immunogen: Synthetic peptide corresponding to the amino terminus of human histone H3 dimethylated at lysine 4.
Histone H3 K4Me2 Antibody Formulation: Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Histone H3 K4Me2 Antibody Quality Control: This antibody has been validated for use in ChIP, IF and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
Is specific for H3 K4Me2
Has no cross-reactivity to related modifications
Is unaffected by nearby modifications
Storage and Stability: Stable for 2 years at -20°C from date of receipt.
Application Notes / Recommended dilutions: ChIP 2 - 5 µg per ChIP IF/IHC 1:50 - 1:200 IP 2 - 5 µg per IP WB 1:500 - 1:2000
View technical datasheet for this product. View additional specificity data for this product.
Peptide Array Specificity Data: EpiTitan™ Histone Peptide Array used to analyze histone modification specificity of Histone H3 K4Me2 Antibody (Lys4 Dimethyl) catalog number 13-0013. Antibody signal appears in red, while the spotting tracer appears in green. (Click image to enlarge)
Chromatin IP Data: Chromatin IP (ChIP) performed on HEK293 cell chromatin using Histone H3 K4Me2 Antibody (black bars) or rabbit IgG (grey bars) as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primers specific for the gene indicated. Data are presented as ratios relative to input DNA in ChIP chromatin. (Click image to enlarge)
Specificity Data-Array: EpiTitan™ Histone Peptide arrays used to analyze the histone modification specificity of Histone H3 K4Me2 Antibody. Data is shown for the highest reacting singly-modified peptides. For more specificity data, see Additional Peptide Array Specificity Data. (Click image to enlarge)
Specificity Data-Dot Blot: Peptide dot blot used to confirm the specificity of Histone H3 K4Me2 Antibody. Methylated peptides corresponding to the immunogen and related sites were spotted onto PVDF and probed with the antibody. (Click image to enlarge)
Immunofluorescence Data: HEK293T cells stained with Histone H3K4me2 Antibody (red) and counterstained with DAPI (blue). (Click image to enlarge)
Western Blot Data: HeLa cell extract (HeLa) and recombinant histone H3 (H3 protein) were blotted onto PVDF and probed with Histone H3 K4Me2 Antibody. (Click image to enlarge)