Histone H3 K27Me1 Peptide Description: A synthetic peptide derived from human histone H3.3, amino acids 15-34, monomethylated at lysine 27. The alpha-amino group on the N-terminus is acetylated, with a PEG spacer containing a biotin moiety at the C-terminus.
Histone H3 K27Me1 Peptide Formulation: 50 µg of lyophilized powder.
Purity: >90% as judged by HPLC and mass spectrometry.
Storage and Stability: Lyophilized peptides are stable for 2 years at -20°C from date of shipment. Peptides in solution are stable for 6 months at -80°C. Spin vial briefly in microfuge and reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot peptide to avoid repeated freezing and thawing.
Quality Control: Peptide purified by reversed-phase HPLC and analyzed using MALD-TOF mass spectrometry and analytical RP-HPLC.
Mass Spec Data: MALDI-TOF mass spectrometry was used to analyze histone H3 K27Me1 peptide for identity and homogeneity.