|Type: Polyclonal ||Host: Rabbit |
|Mol Wgt.: 15 kDa ||Appl.: IF, WB |
|Format: Aff. Pur. IgG ||Reactivity: H, Sc, WR |
Histone H3 K27Me1 Antibody Description:
Rabbit polyclonal antibody recognizing the histone H3 monomethylated at lysine 27. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Methylation of lysine 27 is associated with transcriptional repression by the Polycomb complex of proteins and the formation of facultative heterochromatin.
Histone H3 K27Me1 Antibody Immunogen: Synthetic peptide derived from human histone H3 monomethylated at lysine 27.
Histone H3 K27Me1 Antibody Formulation: Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Histone H3 K27Me1 Antibody Quality Control: This antibody has been validated for use in IF, IHC and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
- Is specific for H3 K27Me1
- Has no cross-reactivity to related modifications
- Is unaffected by nearby modifications
Histone H3 K27Me1 Antibody Storage and Stability: Stable for 2 years at -20°C from date of receipt.
Histone H3 K27Me1 Antibody Application Notes Recommended dilutions:
IF/IHC 1:50 - 1:200
WB 1:500 - 1:2000
Histone H3 K27Me1 Antibody References:
|View technical datasheet for this product. |
View additional specificity data this product.
Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting
Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish