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EpiCypher

Histone H3 K27Me1 Antibody

Cat. No.: 13-0015
List Price: $359.00
Quantity:

Product Description

Type:  Polyclonal Host:  Rabbit
Mol Wgt.:  15 kDa Appl.:  IF, WB
Format:  Aff. Pur. IgG Reactivity:  H, Sc, WR

Histone H3 K27Me1 Antibody Description:

Rabbit polyclonal antibody recognizing the histone H3 monomethylated at lysine 27. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Methylation of lysine 27 is associated with transcriptional repression by the Polycomb complex of proteins and the formation of facultative heterochromatin.

Histone H3 K27Me1 Antibody Immunogen:

Synthetic peptide derived from human histone H3 monomethylated at lysine 27.

Histone H3 K27Me1 Antibody Formulation:

Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.

Histone H3 K27Me1 Antibody Quality Control:

This antibody has been validated for use in IF, IHC and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
  • Is specific for H3 K27Me1
  • Has no cross-reactivity to related modifications
  • Is unaffected by nearby modifications

Histone H3 K27Me1 Antibody Storage and Stability:

Stable for 2 years at -20°C from date of receipt.

Histone H3 K27Me1 Antibody Application Notes

Recommended dilutions:
   IF/IHC  1:50 - 1:200
   WB  1:500 - 1:2000

Histone H3 K27Me1 Antibody References:

View technical datasheet for this product. 13-0015 Datasheet
View additional specificity data this product. 13-0015 Array Spec Data

Applications Key: ChIP-Chromatin IP; E-ELISA; FACS-Flow cytometry; IF-Immunofluorescence; IHC-Immunohistochemistry; IP-Immunoprecipitation; WB-Western Blotting

Reactivity Key: B-Bovine; Ce-C. elegans; Ch-Chicken; Dm- Drosophila; Eu-Eukaryote; H-Human; M-Mouse; Ma-Mammal; R-Rat; Sc-S.cerevesiae; Sp-S. pombe; WR-Wide Range (predicted); X-Xenopus; Z-Zebrafish

13-0015 Epititan Image

Peptide ArraySpecificity Data: EpiTitan? Histone Peptide Array used to analyze histone modification specificity of Histone H3 K27Me1 Antibody (Lys27 monomethyl) catalog number 13-0015. Antibody signal appears in red, while the spotting tracer appears in green.
(Click image to enlarge)

13-0015 Epititan Specificity

Specificity Data-Array: EpiTitan? Histone Peptide arrays used to analyze the histone modification specificity of Histone H3 K27Me1 Antibody. Data is shown for the highest reacting singly-modified peptides. For more specificity data, see Additional Peptide Array Specificity Data.
(Click image to enlarge)

13-0015 Dot Blot

Specificity Data-Dot Blot: Peptide dot blot used to confirm the specificity of Histone H3 K27Me1 Antibody. Methylated peptides corresponding to the immunogen and related sites were spotted onto PVDF and probed with the antibody.
(Click image to enlarge)

13-0015 IF

Immunofluorescence Data: HEK293T cells stained with Histone H3K27Me1 Antibody (red) and counterstained with DAPI (blue).
(Click image to enlarge)

13-0015 Western Blot

Western Blot Data: HeLa cell extract (HeLa) and recombinant histone H3 (H3 protein) were blotted onto PVDF and probed with Histone H3 K27Me1 Antibody.
(Click image to enlarge)