Rabbit polyclonal antibody recognizing the histone H3 monomethylated at lysine 27. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Methylation of lysine 27 is associated with transcriptional repression by the Polycomb complex of proteins and the formation of facultative heterochromatin.
Histone H3 K27Me1 Antibody Immunogen:
Synthetic peptide derived from human histone H3 monomethylated at lysine 27.
Histone H3 K27Me1 Antibody Formulation:
Affinity-purified IgG (1 mg/ml) in PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Histone H3 K27Me1 Antibody Quality Control:
This antibody has been validated for use in IF, IHC and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:
Peptide ArraySpecificity Data: EpiTitan? Histone Peptide Array used to analyze histone modification specificity of Histone H3 K27Me1 Antibody (Lys27 monomethyl) catalog number 13-0015. Antibody signal appears in red, while the spotting tracer appears in green. (Click image to enlarge)
Specificity Data-Array: EpiTitan? Histone Peptide arrays used to analyze the histone modification specificity of Histone H3 K27Me1 Antibody. Data is shown for the highest reacting singly-modified peptides. For more specificity data, see Additional Peptide Array Specificity Data. (Click image to enlarge)
Specificity Data-Dot Blot: Peptide dot blot used to confirm the specificity of Histone H3 K27Me1 Antibody. Methylated peptides corresponding to the immunogen and related sites were spotted onto PVDF and probed with the antibody. (Click image to enlarge)
Immunofluorescence Data: HEK293T cells stained with Histone H3K27Me1 Antibody (red) and counterstained with DAPI (blue). (Click image to enlarge)
Western Blot Data: HeLa cell extract (HeLa) and recombinant histone H3 (H3 protein) were blotted onto PVDF and probed with Histone H3 K27Me1 Antibody. (Click image to enlarge)