SKU: 13-0001
{"url":"https://www.epicypher.com/products/antibodies/ptm-specific-antibodies/histone-h3-c-terminal-antibody","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","options":[],"id":"132","bulk_discount_rates":[],"can_purchase":true,"meta_description":"HIstone H3 C-terminal antibody for use in Chromatin IP and Western, chromatin immunoprecipitation, chip, chip-chip, chip-seq","category":["Epigenetics Kits and Reagents"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/132/645/snap-chip-ab__50489.1560449288.png?c=2","alt":"Histone H3 C-terminal Antibody"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=132","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"923","name":"Pack Size","value":"100 µL"}],"sku":"13-0001","description":"<table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\"><tr> <td valign=\"top\" width=\"600%\"> <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\"> <tbody> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Type: </b>Polyclonal</span></span> </td> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Host: </b>Rabbit</span></span> </td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Mol Wgt.: </b>15 kDa</span></span> </td> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Appl.: </b>ChIP, WB</span></span> </td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Format: </b>Serum</span></span> </td> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Reactivity: </b>Ce, Ch, Dm, H, Sc, WR</span></span> </td> </tr> </tbody> </table> <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\"> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br /> </span></span> <h3><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H3 C-terminal Antibody Description:</b></span></span></h3><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">Rabbit polyclonal antibody recognizing the C-terminus of histone H3, for use in ChIP and Western blot. Histone H3 is one of a core components of the nucleosome, the smallest subunit of chromatin.<br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"> <h3><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H3 C-terminal Antibody Immunogen:</b></span></span></h3><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">Synthetic peptide corresponding the the C-terminus of human histone H3.1, conjugated to KLH.<br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"> <h3><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H3 C-terminal Antibody Formulation:</b></span></span></h3><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">Rabbit serum with 30% glycerol and 0.035% sodium azide.<br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"> <h3><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H3 C-terminal Antibody Quality Control:</b></span></span></h3><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">This antibody has been validated for use in ChIP and Western blot. It has also been validated for specificity by dot blot and histone peptide array and it has been confirmed that this antibody:<br /> </span></span> <ul> <li><span style=\"font-family: Arial; \" class=\"Apple-style-span\">Is specific for H3 C-terminus</span></li> <li><span style=\"font-family: Arial; \" class=\"Apple-style-span\">Has no cross-reactivity to any modifications</span></li> </ul> </td> </tr> <tr> <td valign=\"top\"> <h3><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Histone H3 C-terminal Antibody Storage and Stability:<br /> </span></span></h3><span style=\"font-size: 12pt; font-family: Arial;\">Stable for 2 years at -20°C from date of receipt. </span><br /> <br /> </td> </tr> <tr> <td valign=\"top\"> <h3><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Histone H3 C-terminal Antibody Application Notes:</span></span></h3><span style=\"font-size: 12pt;\" class=\"Apple-style-span\"><b style=\"font-family: Arial;\">Recommended dilutions: </b><br /> <span style=\"font-family: Arial;\"> ChIP 3 - 5 µl per ChIP</span></span><span style=\"font-family: Arial;\" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br /> WB 1:2,500 - 1:5,000<br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"> <h3><span style=\"font-family: Arial; font-size: 16px;\">Histone H3 C-terminal Antibody </span><span style=\"font-size: 12pt; font-family: Arial;\">References:</span></h3><span style=\"font-family: Arial; font-size: 10pt; \"> Rothbart SB et al (2012). <i>Nat Struct Mol Biol</i> 11: 1155-1160. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/23022729\">Link</a><br /> Rizzardi LF et al (2012). <i>Genetics</i> 192: 371-384. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/22851644\">Link</a><br /> Rothbart SB et al (2013). <i>Genes Dev</i> 27: 1288-1298. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/23752590\">Link</a></span><br /> <br /> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial;\" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/13-0001.pdf\" target=\"_blank\"> <img src=\"/content/documents/tds/icon.png\" alt=\"13-0001 Datasheet\" width=\"30\" height=\"40\" /></a><br /> <br /> </span></span> <p><span style=\"font-family: Arial; font-size: 10pt;\"><strong>Applications Key: ChIP</strong>-Chromatin IP; <strong>E</strong>-ELISA; <strong>FACS</strong>-Flow cytometry; <strong>IF</strong>-Immunofluorescence; <strong>IHC</strong>-Immunohistochemistry; <strong>IP</strong>-Immunoprecipitation; <strong>WB</strong>-Western Blotting</span></p> <p><span style=\"font-family: Arial; font-size: 10pt;\"><strong>Reactivity Key: </strong><strong>B</strong>-Bovine; <strong>Ce</strong>-C. elegans; <strong>Ch</strong>-Chicken; <strong>Dm</strong>- Drosophila; <strong>Eu</strong>-Eukaryote; <strong>H</strong>-Human; <strong>M</strong>-Mouse; <strong>Ma</strong>-Mammal; <strong>R</strong>-Rat; <strong>Sc</strong>-S.cerevesiae; <strong>Sp</strong>-S. pombe; <strong>WR</strong>-Wide Range (predicted); <strong>X</strong>-Xenopus; <strong>Z</strong>-Zebrafish</span></p> </td> </tr> </table> </td> <td valign=\"top\"> <table width=\"250px\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\"> <tr> <td align=\"center\"><a> <img src=\"/content/images/products/antibodies/13_0001_wb_tds.jpg\" alt=\"13-0001 WB\" width=\"195\" height=\"350\" /></a> </td> </tr> <tr> <td valign=\"top\" align=\"left\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Western Blot Data: </b>Western blot using Histone H3 C-terminal Antibody (1:5,000 dilution) 0.2 µg of HEK293 core histones.</span></span> </td> </tr> <tr valign=\"top\"> <td valign=\"top\" align=\"center\"><a> <img src=\"/content/images/products/antibodies/13_0001_chip_tds.jpg\" alt=\"13-0001 ChIP\" width=\"249\" height=\"250\" /></a> </td> </tr> <tr> <td valign=\"top\" align=\"left\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>ChIP Data: </b>ChIP using Histone H3 C-terminal Anti-body (4.5 µl per ChIP) with <em>S. cerevesiae</em> chromatin. PCR was performed using <em>GAL10</em> gene primers or telomeric (TEL) control primers. Lane 1: Chromatin taken from cells where <em>GAL10</em> gene is not ex-pressed. Lane 2: <em>GAL10</em> gene expressed. </span></span> </td> </tr> </table></td> </tr> </table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$385.00","value":385,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"HIstone H3 C-terminal antibody for use in Chromatin IP and Western","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"HIstone H3 antibody, C-terminal, Chromatin IP and Western, chip, human, chromatin immunoprecipitation, chip, chip-chip, chip-seq","show_quantity_input":1,"title":"Histone H3 C-terminal Antibody","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/132/645/snap-chip-ab__50489.1560449288.png?c=2","alt":"Histone H3 C-terminal Antibody"}]}
Pack Size: 100 µL
