Histone H3.3, Recombinant Human Description: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) expressed in E. coli and purified by FPLC. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). H3.3-H4 associates in vivo with the HIRA chaperone complex.
Histone H3.3, Recombinant Human Formulation: 100 µg of lyophilized powder.
Histone H3.3, Recombinant Human Storage and Stability: Lyophilized proteins are stable for 2 years at -20°C from date of shipment. Reconstitute with sterile distilled water and aliquot to avoid repeated freezing and thawing. Stable for 6 months at -80°C after reconstitution.
Histone H3.3, Recombinant Human Application Notes: Recombinant histone H3.3 is suitable for enzyme assays and nucleosome reconstitution.
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QC Purity Data: Histone H3.3, Recombinant Human, (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. (Click image to enlarge)