Histone H2AX, Recombinant Human Description: Recombinant human histone H2AX (H2AFX, accession P16104) expressed in E. coli and purified by FPLC. Histone H2AX is a histone variant that is incorporated into a subset of nucleosomes in place of canonical histone H2A. H2AX is phosphorylated on serine 139 by the ATM and ATR kinases in response to DNA double strand breakage. Phosphorylated H2AX serves as a platform to recruit a number of DNA damage repair proteins, including BRCA1, NBS1 and MDC1.
Histone H2AX, Recombinant Human Formulation: 100 µg of lyophilized powder.
Histone H2AX, Recombinant Human Storage and Stability: Lyophilized proteins are stable for 2 years at -20°C from date of shipment. Reconstitute with sterile distilled water and aliquot to avoid repeated freezing and thawing. Stable for 6 months at -80°C after reconstitution.
Histone H2AX, Recombinant Human Application Notes: Recombinant histone H2AX is suitable for enzyme assays and nucleosome reconstitution.
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QC Purity Data: Histone H2AX, Recombinant Human, (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. (Click image to enlarge)