Histone H2A/H2B Dimers, Recombinant Human Description: Histone H2A/H2B dimers made from recombinant histones expressed in E. coli (accession numbers: H2A-P04908; H2B-O60814). Histones H2A and H2B were expressed and purified individually by FPLC, then assembled into dimers that were further purified using gel filtration chromatography.
Histone H2A/H2B Dimers, Recombinant Human Formulation: 50 µg of H2A/H2B dimer (0.5 mg/ml) in 10mM Tris-HCl pH 7.5, 2M NaCl, 1mM EDTA, 2mM DTT, 20% glycerol.
Histone H2A/H2B Dimers, Recombinant Human Storage and Stability: Lyophilized proteins are stable for 2 years at -20°C from date of shipment. Reconstitute with sterile distilled water and aliquot to avoid repeated freezing and thawing. Stable for 6 months at -80°C after reconstitution.
Histone H2A/H2B Dimers, Recombinant Human Application Notes: Recombinant histone H2A/H2B Dimers are suitable for enzyme assays and nucleosome reconstitution.
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QC Protein Data: Histone H2A/H2B Dimers, Recombinant Human, (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. (Click image to enlarge)
QC Purification Data: Chromatogram from gel filtration purification of Histone H2A/H2B Dimer, Recombinant Human. Only fractions corresponding to the intact dimer (as indicated, black bar) were collected and pooled. (Click image to enlarge)