Glutathione S-transferase (GST) is a 26 kDa protein from Schistosoma japonica and is used as an epitope tag for improving the expression and solubility of GST fusion proteins expressed in E coli. This antibody is useful for detection of GST-tagged proteins on the EpiTitan Histone Peptide array and for detection of GST-tagged fusion proteins by Western Blot, including proteins bound to specific peptides in pull-down experiments.
GST Epitope Tag Antibody Immunogen:
Purified recombinant GST from Schistosoma japonica.
GST Epitope Tag Antibody Formulation:
Rabbit serum with 30% glycerol and 0.035% sodium azide.
GST Epitope Tag Antibody Storage and Stability:
Stable for 2 years at -20°C from date of receipt.
GST Epitope Tag Antibody Application Notes
Recommended dilutions: IF 1:500 - 1:2,000 WB 1:1,000 - 1:5,000
GST Epitope Tag Antibody References:
Hacker KE et al (2016). J Biol Chem DOI: 10.1074/jbc.M116.739375 LINK
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Immunofluorescence Data: GST Epitope Tag Antibody (1:1,000 dilution) used to label a GST-tagged fusion protein bound to specific peptides on the EpiTitan Histone Peptide Array (Catalog No. 11-2001). The GST antibody was then detected using an AlexaFluor 647-conjugated secondary antibody (red). (Click image to enlarge)