Epigenetics Services

EpiCypher offers a variety of services to assist you in your epigenetics and chromatin biology research, including histone peptide array screening, peptide pull-down, fluorescence polarization to analyze protein interactions and peptide synthesis.

Histone Modification Interaction Screening and Analysis Services

Histone modifications are important regulators of cell function and genome activity. There are hundreds of histone binding proteins that recognize specific histone modifications and mediate important downstream effects. Antibodies that recognize histone modifications are important tools in chromatin and epigenetics research. Histone modifying enzymes are important in human disease states and targets for therapeutic intervention. EpiCypher can facilitate the characterization of these proteins and antibodies by performing the histone modification screening experiments for you, including histone peptide array screening, enzyme substrate identification and protein binding constant determination. Put our expertise and experience screening histone modifications to work for you. For more information contact us at info@epicypher.com

Histone Peptide Array Screening

EpiCypher’s new EpiTitan™ Histone Peptide Arrays are the best arrays available on the market. Each peptide on the array is synthesized, HPLC purified and verified by mass spectrometry prior to spotting. This significantly reduces false-negatives and false-positives, thereby reducing time and cost of experiments. 

  • Analyze the specificity of histone binding "effector" proteins
  • Examine the specificity of histone modification antibodies
  • Determine the substrate specificity of histone-modifying enzymes

Epigold Histone Peptide Microarray Workflow

Click Image to Enlarge

Histone Peptide Pull-down Services

For some histone binding proteins, histone peptide pull-downs are a more straightforward approach to identify proteins that interact with histone modifications. EpiCypher offers a comprehensive solution for protein-peptide interactions and employ a proprietary protocol that maximizes sensitivity while lowering non-specific background. In brief, your protein is mixed with one of our hundreds of high quality histone peptides and the protein bound to the peptide is analyzed by Western blotting.

  • Peptide pull-downs are extremely sensitive
  • Identification of interactions not detectable using other techniques
  • Includes positive control protein to your modification

Histone Peptide Pulldown Schematic Click Image to Enlarge

Fluorescence Polarization Determination of Dissociation Constant (Kd)

Fluorescence Polarization (FP) is a technique based on the principle of fluorescence anisotropy. Light emitted from a fluorescent molecule (in this case a fluorescently-labeled histone peptide) will be less polarized compared to when bound by a larger molecule, such as a histone binding protein. This difference can be measured and used to calculate the dissociation constant of your protein for a specific modification.

  • Quantitative and reproducible
  • Does not require large amounts of protein
  • Identify Kd for proteins or antibodies

Fluorescence Polarization Schematic Click Image to Enlarge

Please contact info@epicypher.com for additional information and pricing or complete a Request for Quote form.

HeLa Nuclear Extract
HeLa Nuclear Extract Description: HeLa cells are an immortalized human cell line derived from a cervical cancer biopsy. HeLa nuclear extract is an excellent source of transcription factors, histones, chromatin proteins and histone deacetylase (HDAC)...
£110.10
Histone H3.3 Expression Vector pEPI-H3.3WT
Histone H3.3 Expression Vector pEPI-H3.3WT Description: A mammalian vector encoding the expression of human histone H3.3 (accession P84243, molecular weight of 15 kDa) driven by the cytomegalovirus (CMV) promoter, containing a C-terminal DDDDK...
£183.99
Histone H3.3 Expression Vector pEPI-H3.3K9M
Histone H3.3 Expression Vector pEPI-H3.3K9M Description: A mammalian vector encoding the expression of human histone H3.3 (accession P84243, molecular weight of 15 kDa) with a mutation substituting methionine for lysine at position 9, driven by...
£183.99
Histone H3.3 Expression Vector pEPI-H3.3K27M
Histone H3.3 Expression Vector pEPI-H3.3K27M Description: A mammalian vector encoding the expression of human histone H3.3 (accession P84243, molecular weight of 15 kDa) with a mutation substituting methionine for lysine at position 27, driven by...
£183.99
Histone H3 C-terminal Antibody
Type:  Polyclonal Host:  Rabbit Mol Wgt.:  15 kDa Appl.:  ChIP, WB Format:  Serum Reactivity:  Ce, Ch, Dm, H, Sc, WR ...
£265.27
Histone H3 R8Me1 Antibody
Type:  Polyclonal Host:  Rabbit Mol Wgt.:  15 kDa Appl.:  ChIP, IF, IHC, WB Format:  Aff. Pur IgG Reactivity:  H, WR Histone H3 R8Me1 Antibody Description: Rabbit...
£265.27
Histone H3 R8Me2a Antibody
Type:  Polyclonal Host:  Rabbit Mol Wgt.:  15 kDa Appl.:  IF, IHC, IP, WB Format:  Aff. Pur. IgG Reactivity:  H, WR Histone H3 R8Me2a Antibody Description: ...
£265.27
Histone H3 R8Me2s Antibody
Type:  Polyclonal Host:  Rabbit Mol Wgt.:  15 kDa Appl.:  IF, IHC, IP, WB Format:  Aff. Pur. IgG Reactivity:  H, WR Histone H3 R8Me2s Antibody Description: Rabbit...
£265.27
Histone H3 K9Ac Antibody
Type:  Polyclonal Host:  Rabbit Mol Wgt.:  15 kDa Appl.:  ChIP, ChIP-seq, IF, IP, WB Format:  Aff. Pur. IgG Reactivity:  H, Sc, WR Histone H3 K9Ac Antibody...
£265.27
Histone H4 K8Ac Antibody
Type:  Polyclonal Host:  Rabbit Mol Wgt.:  15 kDa Appl.:  ChIP, ChIP-seq, IF, IP, WB Format:  Aff. Pur. IgG Reactivity:  H, Sc, WR Histone H4 K8Ac Antibody...
£265.27