SKU: 16-4101
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"347","bulk_discount_rates":[],"can_purchase":true,"meta_description":"EpiDyne Nucleosome Substrate for Chromatin Remodeling Assays and Inhibitor Screening","category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/347/803/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__47518.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=347","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1053","name":"Pack Size","value":"50 μg"},{"id":"1054","name":"Internal Comment","value":"no more bulk 3/31/22"}],"sku":"16-4101","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <p>\n Mononucleosomes assembled from recombinant human histones expressed\n in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4.\n Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805)\n wrapped by provided 217 base pair DNA sequence that includes the\n Widom 601 sequence with an added GATC.\n </p>\n <p\n style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n \">\n <a\n target=\"_blank\"\n style=\"color: #fff\"\n href=\"/resources/protocols/epidyne-guide\"\n >Want to learn more? Check out our EpiDyne® Guide >>\n </a>\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-4101_protein_gel_tds.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-4101 DNA Gel\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_DNA_gel_tds.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA Gel Data </strong><br />Free DNA\n (DNA, 200 ng) and EpiDyne Nucleosome Remodeling Assay\n Substrate ST601-GATC1 (Nuc, 370 ng) run on an acrylamide gel\n and stained with ethidium bromide to visualize DNA.\n <strong\n >Note absence of free DNA in "Nuc", indicating\n all DNA complexed with histones.</strong\n >\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"content/images/products/nucleosomes/16-4101_DNA_gel_tds.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-4101 Protein Gel\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_protein_gel_tds.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein Gel Data </strong><br />\n Coomassie stained PAGE gel of proteins in EpiDyne Nucleosome\n Remodeling Assay Substrate ST601-GATC1 (2 µg) demonstrates\n the purity of the histones in the preparation. Sizes of\n molecular weight markers and positions of the core histones\n (H2A, H2B, H3 and H4) are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"content/images/products/nucleosomes/16-4101_remodel_tds.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n alt=\"16-4101 Western Blot\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_remodel_tds.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Nucleosome Remodeling Data </strong><br />\n RSC/ATP-dependent chromatin remodeling reaction in the\n presence of DpnII restriction enzyme. EpiDyne Nucleosome\n Remodeling Assay Substrate ST601-GATC1 was incubated with\n RSC chromatin remodeling complex (10 nm) or without (No RSC)\n for up to two hours in the presence of 2 mM ATP (or No ATP,\n as indicated) and 50U of DpnII. Samples were quenched at\n specified intervals and run on 8% native PAGE gel and\n stained with ethidium bromide. The apperance of a band at\n ~200 base pairs (red arrow) indicates cleavage at GATC\n subsequent to chromatin remodeling.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"16-0009 DNA Gel\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_DNA_gel_tds.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n alt=\"16-0009 Protein Gel\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_protein_gel_tds.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img\n alt=\"16-4101 Western Blot\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_remodel_tds.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p>\n Purified recombinant mononucleosomes 1.70 mg/ml (DNA + protein\n weight) in 29.4 µl of 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM\n EDTA, 2 mM DTT, 20% glycerol. MW = 242,900 Da. Molarity = 7.00\n µM.\n </p>\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six (6) months at -80°C from date of receipt. For\n best results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n This product is a substrate for chromatin remodeling assays (<em\n >e.g.</em\n >\n <a href=\"/acf-remodeling-enzyme-complex/\" target=\"_new\"\n >ACF Remodeling Enzyme Complex</a\n >) using restriction enzymes to determine accessibility of GATC\n subsequent to chromatin remodeling.\n </p>\n </div>\n </div>\n </div>\n<div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">DNA Sequence</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p style=\"line-break: anywhere;\">\n CTAGGAATTCATCAGAATCCCGGTGCCGAGGCCGATCAATTGGTCG\n TAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCG\n CGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGT\n CAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGA\n TGGATGATGATGGATGAATAGATGGATGGATGAAGCTTTAGG\n </p>\n \n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Product References:</strong>\n <iframe\n class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shrVZ2ecXmqA8RE03?backgroundColor=purple&viewControls=on\"\n frameborder=\"0\"\n onmousewheel=\"\"\n width=\"100%\"\n height=\"300\"\n style=\"background: transparent; border: 1px solid #ccc\"></iframe>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-4101.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/technotes/EpiDyne_REA.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >Restriction Enzyme Assay application technical note for this\n product</span\n >\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$565.00","value":565,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"EpiDyne Nucleosome Substrate ST601-GATC1 | For Chromatin Remodeling Assays and Inhibitor Screening","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"options":[],"related_products":[{"id":551,"sku":"15-1013","name":"ACF Chromatin Remodeling Enzyme Complex","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"summary":"\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2","alt":"ACF Chromatin Remodeling Enzyme Complex"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2","alt":"ACF Chromatin Remodeling Enzyme Complex"}],"date_added":"24th Jul 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":585,"name":"Pack Size","value":"100 reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=551","price":{"without_tax":{"currency":"USD","formatted":"$505.00","value":505},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=551"},{"id":386,"sku":"16-4201","name":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate","url":"https://www.epicypher.com/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":"\n \n \n \n Description\n \n \n This product provides a h","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/386/276/2_EpiDyne__20008.1569012494.png?c=2","alt":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/386/276/2_EpiDyne__20008.1569012494.png?c=2","alt":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate"}],"date_added":"28th Aug 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1138,"name":"Pack Size","value":"50 µg"},{"id":1139,"name":"Internal Comment","value":"Bulk in Bulk Nuc Box 2 in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.00 LBS","value":0},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=386","price":{"without_tax":{"currency":"USD","formatted":"$595.00","value":595},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=386"},{"id":470,"sku":"16-4111","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":" \n\n \n\n \n\n\n\n EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Description: 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Pack Size: 50 μg
