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EpiCypher

ENL YEATS Domain, Recombinant Human

Cat. No.: 15-0069
List Price: $299.00
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Product Description

Type:  YEATS Host:  E. coli
Mol Wgt.:  19 kDa Epitope Tag:  His

ENL YEATS Domain, Recombinant Human Description: ENL YEATS Domain, Recombinant Human (MLLT1, LTG19, YEATS1, accession Q03111, amino acids 1 to 150) containing an N-terminal GST tag, expressed in E. coli. The ENL protein is part of the super elongation complex regulating RNA Pol II transcription. A chromosomal translocation resulting in an MLL-ENL fusion is involved in pediatric Acute Lymphoblastic Leukemia. ENL YEATS Domain, Recombinant Human interacts specifically with histone H3 crotonylated at lysine 27 and to a lesser extent lysine 9.

ENL YEATS Domain, Recombinant Human Formulation: Recombinant 6xHis-tagged protein at 1 mg/ml in 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 20% glycerol.

ENL YEATS Domain, Recombinant Human Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

ENL YEATS Domain, Recombinant Human Application Notes: ENL YEATS Domain, Recombinant Human is useful for protein binding and screening experiments examining crotonylated and acetylated protein substrates.

References Using this Product:



View technical datasheet for this product. 15-0069 Datasheet

View ENL Acyl-modified histone binding tech note. ENL Tech Note

15-0069 Protein Gel Data

Protein Gel Data: ENL YEATS Domain, Recombinant Human (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.
(Click image to enlarge)

15-0069 Protein Interaction Data

Protein Interaction Data: ENL YEATS Domain, Recombinant Human used in peptide binding studies to detect interactions with specific post-translational modifications using AlphaLISA. The identities of the peptides tested are listed on the X-axis.
ac-acetylated
cr-crotonylated
(Click image to enlarge)