Recombinant BRDT Bromodomain 2 Description: Recombinant human BRDT bromodomain 2 (CT9, Fsrg3, accession number Q58F21, amino acids 258 to 387) containing an N-terminal GST tag, expressed in E. coli. BRDT is a testis-specific bromodomain protein that binds histone H4 acetylated at lysine 5 and lysine 8. BRDT is required for spermatogenesis and male mice lacking BRDT are sterile. BRDT is expressed in a percentage of cancers such as non-small cell lung cancer and squamous cell carcinomas of the head, neck and esophagus.
Recombinant BRDT Bromodomain 2 Formulation: Recombinant GST-tagged protein at 1 mg/ml in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 20% glycerol.
Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Application Notes: Recombinant BRDT Bromodomain 2 is useful for protein binding and screening experiments examining acetylated protein substrates.
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Protein Gel Data: BRDT Bromodomain 2 (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. (Click image to enlarge)