Recombinant BRD3 Bromodomain 1 Description: Recombinant human BRD3 Bromodomain 1 (RING3L, accession number Q15059, amino acids 25 to 160) containing an N-terminal GST tag, expressed in E. coli. BRD3 is a member of the BET sub-family of bromodomain-containing proteins, which also includes BRD2, BRD4 and BRDT. BET proteins have two bromodomains and an ET domain C-terminal to the second bromodomain. BRD3 interacts with acetylated histone and non-histone proteins, including acetylated GATA1. BRD3 is found fused to the NUT protein in a subset of carcinomas, contributing to the oncogenic phenotype by suppressing differentiation.
Recombinant BRD3 Bromodomain 1 Formulation: Recombinant GST-tagged protein at 1 mg/ml in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 20% glycerol.
Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Application Notes: Recombinant BRD3 Bromodomain 1 is useful for protein binding and screening experiments examining acetylated protein substrates.
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Protein Gel Data: BRD3 Bromodomain 1 (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. (Click image to enlarge)