Recombinant BRD2 Bromodomain 1 Description: Recombinant human BRD2 Bromodomain 1 (RING3, accession number P25440, amino acids 57 to 200) containing an N-terminal GST tag, expressed in E. coli. BRD2 is a member of the BET sub-family of bromodomain-containing proteins, which also includes BRD3, BRD4 and BRDT. BET proteins have two bromodomains and an ET domain C-terminal to the second bromodomain. BRD2 has been reported to bind multiple sites of acetylation on histone H4. BRD2 is essential for mammalian development, and has been linked to Juvenile Myoclonic Epilepsy.
Recombinant BRD2 Bromodomain 1 Formulation: Recombinant GST-tagged protein at 1 mg/ml in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 20% glycerol.
Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Application Notes: Recombinant BRD2 Bromodomain 1 is useful for protein binding and screening experiments examining acetylated protein substrates.
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Protein Gel Data: BRD2 Bromodomain 1 (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. (Click image to enlarge)